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园艺学报 ›› 2016, Vol. 43 ›› Issue (2): 218-226.doi: 10.16420/j.issn.0513-353x.2015-0442

• 果树 • 上一篇    下一篇

桃钾转运体基因PpeKUP5的表达及功能分析

宋志忠,马瑞娟*,郭绍雷,俞明亮,许建兰   

  1. 江苏省农业科学院园艺研究所,江苏省高效园艺作物遗传改良实验室,南京 210014
  • 出版日期:2016-02-25 发布日期:2016-02-25
  • 基金资助:
    国家自然科学基金项目(31501743);国家现代农业产业技术体系建设专项资金项目(CARS-31);江苏省农业科技自主创新基金项目[CX(14)2015,CX(15)1020]

Expression and Function Analysis of Potassium Transporter Gene PpeKUP5 in Peach

SONG Zhi-zhong,MA Rui-juan*,GUO Shao-lei,YU Ming-liang,and XU Jian-lan   

  1. Institute of HorticultureJiangsu Academy of Agricultural SciencesJiangsu Key Laboratory of Horticultural Crop Genetic ImprovementNanjing 210014China
  • Online:2016-02-25 Published:2016-02-25

摘要: 从‘霞晖8号’桃中克隆了一个定位于第4条染色体上的钾转运体基因PpeKUP5,该基因编码625个氨基酸,具有10个跨膜结构域;PpeKUP5主要在根部表达,其次是叶和茎中,花和果实中的表达量极低;ABA、重金属Cr和Zn处理均显著诱导其在桃幼苗各组织中的表达,其中Cr处理最为显著,高钾胁迫及重金属Cu处理显著降低了其在根部的表达水平;细菌互补试验表明PpeKUP5具有吸收外界K+(KCl或K2SO4)的功能,且在偏中性pH值条件下最为显著。本研究表明PpeKUP5是一个主导桃树根部K+吸收的钾转运体,并可能在桃树适应高钾及重金属Cr、Zn和Cu等胁迫处理和ABA 响应中起着重要作用。

关键词: 桃, 钾转运体, PpeKUP5基因, 基因表达, 功能验证

Abstract: An K+ transporter encoding gene(PpeKUP5)was isolated from‘Xiahui 8’peach(Prunus persica)trees. The PpeKUP5 encodes a protein of 625 amino acid residues,which contains 10 transmembrane regions. The PpeKUP5 gene was largely expressed in roots,less in leaves and stems,and very weakly in flowers and fruits. Abscisic acid(ABA)treatment,heavy-metal chromium(Cr)and heavy-metal zinc(Zn),respectively,significantly enhanced the expression level of PpeKUP5 gene throughout whole peach seedlings. Notably,PpeKUP5 gene was most sensitive to Cr treatment. While both K+ excess and heavy-metal copper(Cu)treatment significantly reduced the expression level of PpeKUP5 gene in plant roots. Functional complementation of bacterial mutant showed that PpeKUP5 can utilize external K+(either KCl or K2SO4),especially under neutral pH values. This study favorably reveals that PpeKUP5 is a key K+ transporter mediates roots K+ uptake and accumulation in peach,which may play an important role in response to K+ excess,heavy-metal and ABA stresses.

Key words: peach, potassium transporter, PpeKUP5 gene, gene expression, functional validation

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