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园艺学报 ›› 2015, Vol. 42 ›› Issue (10): 1993-2001.doi: 10.16420/j.issn.0513-353x.2015-0084

• 观赏植物 • 上一篇    下一篇

春兰炭疽病致病菌Colletotrichum boninense的鉴定及生物学特性分析

周平兰1,4,唐新科4,龙岳林2,*,周小毛2,3,*   

  1. 1湖南农业大学园艺园林学院,长沙 410128;2湖南农业大学植物保护学院,长沙 410128;3湖南省农业科学院农业生物技术研究中心,长沙 410125;4湖南科技大学生命科学学院,湖南湘潭 411201
  • 出版日期:2015-10-25 发布日期:2015-10-25
  • 基金资助:

    湖南省科技厅重点项目(2010NK2007)

Identification of Pathogen Colletotrichum boninense from Cymbidium goeringii and Its Biological Characteristics

ZHOU Ping-lan1,4,TANG Xin-ke4,LONG Yue-lin2,*,and ZHOU Xiao-mao2,3,*   

  1. 1College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;2College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;3Center of Biotechnology Research,Hunan Academy of Agricultural Sciences,Changsha 410125,China;4School of Life Science,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China
  • Online:2015-10-25 Published:2015-10-25

摘要:

以湖南野生春兰(Cymbidium goeringii)引种驯化过程中发生的炭疽病病株为试验材料,分离、鉴定病原菌,分析营养因子和非营养因子对病原菌生长的影响。结果表明:春兰炭疽病病原菌为博宁炭疽菌(Colletotrichum boninense Moriwaki,Sato & Tsukiboshi);适合生长的培养基有PDA、PSA和CSA;能有效利用多种碳源,最适合的为葡萄糖,有利生长的有机氮为酵母膏和蛋白胨,无机氮为硝酸钾;光照与黑暗对菌丝生长的影响无显著性差异;适宜pH 5.0 ~ 8.0;在10 ~ 35 ℃均能生长,最适温度为25 ℃;处理20 min,菌丝和孢子的致死温度分别为48和50 ℃。

关键词: 春兰, 炭疽病, Colletotrichum boninense, 生物学特性

Abstract:

Pathogen causing anthracnose on Cymbidium goeringii in Hunan was isolated and identified,and the impact of nutritional and non-nutritional factors on its growth was analyzed. The results showed that the pathogen of anthracnose on Cymbidium goeringii was Colletotrichum boninense Moriwaki,Sato & Tsukiboshi. This strain grew well on media of PDA,PSA and CSA. For carbon sources,it grew better in glucose than in sucrose,maltose or fructose. Yeast extract,peptone and KNO3 were the better organic and inorganic nitrogen sources,respectively. The optimal acidity of culture medium was pH 5.0–8.0,light or darkness. Its hyphae could extend at 15–35 ℃,and the optimum temperature was 25 ℃. The lethal temperatures of hyphae and spores were 48 ℃ and 50 ℃ in 20 min,respectively.

Key words: Cymbidium goeringii, anthracnose, Colletotrichum boninense, biological characteristic

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