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园艺学报 ›› 2015, Vol. 42 ›› Issue (5): 833-842.doi: 10.16420/j.issn.0513-353x.2014-1002

• 果树 • 上一篇    下一篇

桃GH3基因家族的生物信息学分析及其在果实发育中的表达

曾文芳, 潘磊, 牛良, 鲁振华, 崔国朝, 王志强   

  1. 中国农业科学院郑州果树研究所,国家桃葡萄改良中心,农业部果树育种技术重点实验室,郑州 450009
  • 出版日期:2015-05-25 发布日期:2015-05-25
  • 基金资助:

    国家‘863’计划项目(2011AA10020606);国家科技支撑计划项目(2013BAD02B03-2;2014BAD16B04);中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2015-ZFRI)

Bioinformatics Analysis and Expression of the Nectarine Indole-3-acetic- acid-amido Synthase(GH3)Gene Family During Fruit Development

ZENG Wen-Fang, PAN Lei, NIU Liang, LU Zhen-Hua, CUI Guo-Chao, WANG Zhi-Qiang   

  1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,National Peach and Grape Improvement Center,Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture,Zhengzhou 450009,China
  • Online:2015-05-25 Published:2015-05-25

摘要: 为了探索生长素酰胺合成酶(GH3)基因家族在桃(Prunus persica L. Batsch)果实生长发育过程中的作用,对桃基因组中的GH3基因家族进行生物信息学分析,对它们在溶质型和硬质型油桃果实发育成熟阶段的表达水平进行qRT-PCR检测。检测结果表明:桃GH3基因家族有8个成员,分别为PpGH3-1 ~ PpGH3-8,聚类分析表明其分为GroupⅠ和GroupⅡ两类。实时荧光定量PCR分析表明GroupⅡ型基因(PpGH3-1、PpGH3-2、PpGH3-3、PpGH3-4和PpGH3-5)在溶质型油桃中的表达水平高于硬质型油桃,其中PpGH3-3和PpGH3-4最为明显。经NAA处理后的硬质型油桃果实PpGH3-1、PpGH3-3、PpGH3-4和PpGH3-7相比对照上调表达,其中PpGH3-3上调表达最为明显。试验结果表明生长素在桃果实成熟软化过程扮演着重要的角色。

关键词: 桃, 油桃, 生长素酰胺合成酶, 果实发育和成熟, 生长素

Abstract: To explore the role of GH3 gene family during peach fruit development,the members of GH3 gene family were identified and analyzed by bioinformatics;Additionally,the expression levels of GH3 gene were detected in melting-flesh and stony-hard nectarine fruits during fruit development and ripening,as well as in stony-hard nectarine fruits under NAA treatment. The results showed that eight GH3 genes were identified in the peach genome and named as PpGH3-1–PpGH3-8,respectively. Phylogenetic analysis indicated that peach GH3 gene family was divided into two groups,GroupⅠand GroupⅡ. The results of qRT-PCR showed that the expression levels of GH3 genes from GroupⅡ(PpGH3-1,PpGH3-2,PpGH3-3,PpGH3-4 and PpGH3-5)in melting-flesh nectarine fruits were higher than in stony-hard nectarine fruits,especially for PpGH3-3 and PpGH3-4. The expression levels of four genes(PpGH3-1,PpGH3-3,PpGH3-4,and PpGH3-7)in stony-hard nectarine fruits were up-regulated under NAA treatment,especially for PpGH3-3. The results suggested that auxin might play an important role in regulating peach fruit ripening.

Key words: peach, nectarine, GH3(Indole-3-acetic-acid-amido synthase), fruit development and ripening, indole-3-acetic-acid

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