关键词: 番茄, 番茄黄化曲叶病, 遗传分析, 分子标记, 连锁图谱

Abstract:

Genetic analysis and gene mapping were carried out on which is resistant to Tomato yellow leaf curl virus using tomato（Solanum lycopersicum L.）‘13072’（resistant materials）and‘13493’（susceptible materials）as experimental materials in this study. The results showed that a recessive nuclear gene，ty-5，controls the resistance to Tomato yellow leaf curl virus. Simple sequence repeat（SSR）markers were used to conduct on the resistant 657 F2 plants by bulked segregant analysis（BSA）method，and resulted in 5 SSR markers linked to the ty-5 gene. The ty-5 gene was mapped to a linkage group corresponding to chromosome（Chr. 4）of tomato. The flanking markers TES2461 and TGS4151 were linked to the ty-5 gene with genetic distances of 2.4 cM and 3.1 cM，respectively. The physical distance between markers TES2461 and TGS4151 was 737 kb based on the whole genome sequence including 52 candidate genes in this region.

Key words: tomato, Tomato yellow leaf curl virus, genetic analysis, SSR marker, gene mapping