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园艺学报 ›› 2015, Vol. 42 ›› Issue (3): 505-512.doi: 10.16420/j.issn.0513-353x.2014-0900

• 蔬菜 • 上一篇    下一篇

洋葱苯丙氨酸解氨酶基因AcPAL2的克隆与表达分析

梁 毅,刘小义,张洪伟,谭武平   

  1. 1北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京京研益农科技发展中心,北京100097;2中国质量认证中心,北京100070
  • 出版日期:2015-03-25 发布日期:2015-03-25
  • 基金资助:

    国家公益性行业科技项目(20093018);‘十二五’国家科技支撑计划项目(2012BAD02B00,2011BAD35B07,2012BAD50G01);北京市科委育种平台三期项目(D111100001311002);北京市常规育种财政专项(2014-509)

Molecular Cloning and Expression Analysis of AcPAL2 in Onion

LIANG Yi1,LIU Xiao-yi2,ZHANG Hong-wei1,*,and TAN Wu-ping1   

  1. 1Beijing Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China,Beijing Jing Yan Yi Nong Sci-Tech Development Center,Beijing 100097,China;2China Quality Certification Center,Beijing 100070,China
  • Online:2015-03-25 Published:2015-03-25

摘要: 利用简并PCR技术和RACE技术克隆得到了一条洋葱的苯丙氨酸解氨酶(PAL)基因全长cDNA序列。该cDNA序列全长2 349 bp,编码长685个氨基酸残基的多肽序列,命名为AcPAL2。Blast分析表明该序列与虎眼万年青Galtonia saundersiae、野蕉Musa balbisiana的相似性均较高。Real-time PCR表达及花青素含量分析表明,红皮洋葱该基因表达量最大,而黄皮和白皮洋葱表达量极低;在红皮洋葱中该基因在膨大初期大量表达,并迅速降低至一定程度后趋于相对平稳表达,且与花青素的积累过程相一致。

关键词: 洋葱, 花青素, AcPAL2, 基因表达

Abstract: A full-length cDNA of phenylalanine ammonia lyase-homologue(AcPAL2)was isolated from onion using degenerate PCR and the RACE(rapid amplification of cDNA ends)method. The full-length cDNA is 2 349 bp long,which encodes a polypeptide of 685 amino acids. Blast analysis indicate that the polypeptide shares a relatively high similarity with Galtonia saundersiae and Musa balbisiana. Real-time PCR analysis demonstrate that AcPAL2 mRNA express abundantly in red onions,but extremely low in yellow and white onions;AcPAL2 in different bulb swelling stage in red onion has a high expression during the initiation,then express stable after a rapid decrease. And anthocyanin content change also shows the same tendency with AcPAL2 expression.

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