Abstract:

Cytoplasmic male sterile line in Onion (Allium cepa L.) is crucial material for production of hybrid seeds. It is important to identify its cytoplasm and origination, for these could give breeder some guidance suggestions. In this paper, two sets of cytoplasmic male sterile lines, came from two different Chinese onion cultivars, were investigated by molecular method, and were discussed about their polymorphisms with corresponding maintainers. The result showed that the cytoplasms of male sterile lines origin from ‘Sha Gou Hong Pi’and ‘Shuo Zhou Zi Pi’are T and S, respectively. RAPD amplification showed that the S cytoplasm has more polymorphism than T cytoplasm compared to their maintainer lines.

Key words: onion, cytoplasmic male sterile line, identification, polymorphisms