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ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (8): 1563-1572.

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Overexpression of ChPSY Gene from Cerasus humilis Improved Carotenoids Synthesis in Transgenic Tomato

(1山西农业大学园艺学院,山西太谷 030801;2山西省农业科学院现代农业研究中心,太原 030031)   

  1. (1College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China;2Research Centre of Modern Agriculture,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
  • Received:2014-04-16 Online:2014-08-25 Published:2014-08-25

Abstract: The ChPSY cDNA from the Cerasus humilis fruit was analyzed,digested and inserted into plant expression vector PMV,resulting in the plant recombinant vector pBI-ChPSY,which was introduced into tomato via Agrobacterium-mediated transformation. Twelve putatively independent transgenic plants were generated. PCR and Southern blot analysis showed 8 out of these putative transgenic lines were positive,which revealed that ChPSY gene was integrated into the transgenic lines genome. RT-PCR analysis indicated that ChPSY gene was expressed in the fruits of these transgenic lines. HPLC analysis showed that the total amount of carotenoids in the fruit of transgenic tomato was 1.62 to 3.04-fold higher than the non-transformed controls,at the same time,phytoene,lycopene,β-carotene and α-carotene levels were also increased by 4.87,2.10,2.59 and 3.25 folds,respectively. In addition,the expression of endogenous genes involved in carotenoid synthesis was affected in the transgenic lines. These results suggested that overexpression of ChPSY significantly enhanced the carotenoids biosynthesis and accumulation in transgenic tomato fruits.

Key words: Cerasus humilis, tomato, phytoene synthease gene, genetic transformation, carotenoids

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