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ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (5): 889-906.

• Vegetables • Previous Articles     Next Articles

Functional Characterization of ClNLR Gene Involved in Tomato Defense
Against Tomato Yellow Leaf Curl Disease

  

  1. 1Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2Provincial Key Laboratory of Agro-biology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
  • Online:2014-05-25 Published:2014-05-25

Abstract: The previous comparative transcriptome revealed the NBS-LRRs gene(Solyc05g009760.1)
was up-regulated in the TYLCV resistant tomato line CLN2777A and may involve in the resistance to
TYLCV. In this study,the cDNA of this NBS-LRRs gene was isolated from CLN2777A by RT-PCR and
named as ClNLR. Quantative RT-PCR revealed that ClNLR gene was strongly expressed in the root and
leaves of tomato. The transient expression of ClNLR gene in leaves of Nicotiana benthamiana triggered the
hypersensitive reaction(HR). And the virus induced gene silencing(VIGS)of ClNLR gene in the
CLN2777A plantlets facilitated the proliferation of TYLCV as shown by a negative relationship between
the DNA content of TYLCV and the expression levels of ClNLR gene in the VIGS treated plantlets. Taken together,ClNLR gene may be a TYLCV resistance-related gene in tomato.

Key words: tomato, Tomato yellow leaf curl virus, NBS-LRR, gene clone, expression analysis, hypersensitive reaction, gene silence

CLC Number: