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ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (11): 2265-2270.

• Research Notes • Previous Articles     Next Articles

Haploid Plant Induction via in Vitro Gynogenesis and Plant Regeneration in Onion(Allium cepa L.)

LIU  Bing-Jiang, MIAO  Jun, HUO  Yu-Meng, YANG  Yan-Yan, XU  Kun, WU  Xiong   

  1. (1College of Horticultural Sciences and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China;2Vegetable Research Institute of Shandong Academy of Agricultural Sciences,Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province,National Improvement Center for Vegetable,Shandong Branch,Ji’nan 250100,China)
  • Online:2012-11-25 Published:2012-11-25

Abstract: The flower buds of three intermediate-day hybrid cultivars of onion(Allium cepa L.)from Japanese seed company were cultured in vitro using B5 medium which supplied with 100 g · L-1 sucrose and 2,4-D and 6-BA. The suitable media for haploid induction of onion were B5 medium with 1.5 mg · L-1 2,4-D + 1.5 mg · L-1 6-BA and B5 medium with 2.0 mg · L-1 2,4-D + 2.0 mg · L-1 6-BA. The highest induction rate was 4.00%. Forty-three gynogenic embryos were obtained and each of them developed into the whole plant on the B5 medium with no any plant growth regulators. The regenerated plantlets were domesticated and transplanted in greenhouse. The survival rate was 100%. Ploidy analysis of the regenerants with chromosome counts of root tip cells and flow cytometry analysis of leaf tissue revealed that 42 of them are haploid.

Key words: onion, Allium cepa L., gynogenesis, haploid, flow cytometry

CLC Number: