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ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (7): 1380-.

• Research Notes • Previous Articles     Next Articles

Establishment of High-frequency in Vitro Regeneration System of Onion

WANG  Jian-Jun, LIU  Zhao-Kun, HOU  Xi-Lin, YANG  Xue-Dong, KONG   Min, LI  Man-Tang   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Ministry of Agriculture,Nanjing 210095,China
  • Online:2012-07-25 Published:2012-07-25

Abstract: With the young umbels and buds of onion(Allium cepa L.)at different development stage,a high frequency regeneration system of onion in vitro was established by analysis the effects of explant type and plant growth regulator combination on callus induction,shoot regeneration and rooting. The optimum condition was showed as with young umbels on B5 medium with 1.0 mg · L-1 2,4-D + 1.0 mg · L-1 6-BA,with the callus induction rate reaching 100%. The optimum hormone concentration of getting high-frequency regeneracy shoot,with 78.13%,from callus was 1.0 mg · L-1 thidiazuron(TDZ)on B5 medium. All shoots rooted on 1/2MS medium with 0.01 mg · L-1 NAA,the regenerated plantlet were domesticated and transplanted in greenhouse. The survival rate was 95%.

Key words: onion, young umbel, tissue culture, regeneration, thidiazuron(TDZ)

CLC Number: