Studies on Structure and Expression Pattern of Chimeric Genes Associated with Onion T-type Cytoplasmic Male Sterility

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (4): 631-636.

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Studies on Structure and Expression Pattern of Chimeric Genes Associated with Onion T-type Cytoplasmic Male Sterility

WU Hai-tao1,3，WANG Jian-jun1,2，HOU Xi-lin1,2,*，LIU Hong-jiong3，and MA Rong-li3

（1State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agriculture University，Nanjing 210095，China；2Key Laboratory of Southern Vegetable Crop Genetic Improve，Ministry of Agriculture，Nanjing 210095，China；3Vegetable Institute，Shanxi Academy of Agricultural Sciences，Taiyuan 030031，China）

Received:2010-01-15 Revised:2010-03-25 Online:2010-04-25 Published:2010-04-25
Contact: HOU Xi-lin

Abstract

Abstract: The perfect coincidence of orfA501-marker and the phenotype of onion cytoplasmic male sterile has arose interesting in researchers. In this experiment，flanking sequences of orfA501 in mitochondrial genome of onion T-type cytoplasmic male sterility was obtained successfully by anchored PCR，and expression pattern of the chimeric gene was analysed by real time PCR. The result showed that：It was cytochrome c oxidase subunit I（coxⅠ）gene who flanked 5′ of orfA501，and a complicated sequence composed by fractions of atp9，atp6，atp1 and nad4 located in the 3′ region of orfA501. Though there were 93 bases between orfA501 and coxⅠ，they transcripted seperately in the anthors of T-type cytoplasmic male sterility，and orfA501 seemed to play an up-regulator factor for the transcription of coxⅠ.

Key words: onion, Allium cepa L., cytoplasmic male sterility, orfA501

CLC Number:

S 633.2

WU Hai-tao;WANG Jian-jun;HOU Xi-lin;;LIU Hong-jiong;and MA Rong-li. Studies on Structure and Expression Pattern of Chimeric Genes Associated with Onion T-type Cytoplasmic Male Sterility[J]. ACTA HORTICULTURAE SINICA, 2010, 37(4): 631-636.

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Studies on Structure and Expression Pattern of Chimeric Genes Associated with Onion T-type Cytoplasmic Male Sterility

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