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ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (9): 1311-1316.

• 蔬菜 • Previous Articles     Next Articles

Differential Expression Analysis in Flower Buds of CMS and Its Maintaner Line of Capsicum annuum L.

WU Zhi-ming1,2;HU Kai-lin2*;QIAO Ai-min1   

  1. (1College of Agriculture and Landscape A rchitecture, Zhongkai University of Agricultural and Engineering, Guangzhou 510225,China; 2College of Horticulture, South China Agricultural University,Guangzhou 510642, China)
  • Received:2009-04-15 Revised:2009-08-11 Online:2009-09-25 Published:2009-09-25
  • Contact: HU Kai-lin

Abstract: In order to understand the mechanism of CMS in hot pepper, the gene exp ression in flower buds of CMS line and its maintainer line was compared by means of cDNA-AFLP. A total of 19 differential transcript derived fragments ( TDFS ) were identified and sequenced. Four TDFswere exp ressed specifically in CMS line, whereas 15 others could be detected only in the maintainer line. The analysis of sequence homology and functional classification indicated that three TDFS coming from CMS line shared high identities with mitochondrial or ribosomal RNA genes of tomato and tobacco. 12 TDFS from maintainer line were highly homologous to known sequences. These genesmight be related to transcrip tion, defense response, electron transport and energy pathway, DNA or RNA-mediated metabolism, protein metabolism, transport, unknown or putative protein and so on. It is speculated that the occurrence of CMS in pepper might involve different metabolism path ways.

Key words: Capsicum annuum L., CMS, gene expression, cDNA-AFLP

CLC Number: