Isolation of Fertility Restoration-related ESTs in Pepper Cytopla smic MaleSterility Lines Using SSH

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (10): 1443-1449.

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Isolation of Fertility Restoration-related ESTs in Pepper Cytopla smic MaleSterility Lines Using SSH

GUO Shuang;SHEN Huo-lin*; YANG Wen-cai, YANG Juan;WANG Wen

(College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193,China)

Received:2009-04-30 Revised:2009-07-06 Online:2009-10-25 Published:2009-10-25
Contact: SHEN Huo-lin

Abstract

Abstract: To isolate fertility restoration-related genes in pepper cytopasmic male sterility (CMS) lines, a forward subtracted cDNA librarywas constructed using supp ression subtractive hybridization (SSH).The cDNA of pepper CMS line 23A and 121A were used as driver and near2isogenic restorer line 23C and 121C as tester. Genes differentially exp ressed were screened using dot blot hybridization. A total of 175 non-redundant expressed sequence tags (ESTs) were identified. The genesppotential function analysis showed that 103 of these genes were homologous to known genes, and 55 could be new genes. The ESTs were sorted into 14 functional categories according to the Munich Information Center for Protein Sequences (MIPS) method.

Key words: pepper, cytoplasmicmale sterility, restorer gene, suppression subtractive hybridization, differential expression

CLC Number:

S 641.3

GUO Shuang;SHEN Huo-lin;YANG Wen-cai;YANG Juan;WANG Wen. Isolation of Fertility Restoration-related ESTs in Pepper Cytopla smic MaleSterility Lines Using SSH[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1443-1449.

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Isolation of Fertility Restoration-related ESTs in Pepper Cytopla smic MaleSterility Lines Using SSH

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